SARIB

Partner No 3: Rudjer Bošković Institute (RBI)

The Ruđer Bošković Institute (RBI) was founded in 1951in Zagreb and is the largest multidisciplinary and interdisciplinary research institution in Croatia. RBI has almost 750 employees with about 350 senior (Ph.D.) scientists and 200 Ph.D. students. The basic mission of the Institute is the fundamental research in natural sciences (physics, chemistry, biology and biochemistry), material, medical and biomedical sciences, marine and environmental sciences. Biomedical research and molecular genetics have recently acquired considerable attention as a promising avenue for interdisciplinary and applied research. Research activities are organized within 12 divisions: Theoretical Physics, Experimental Physics, Materials Physics, Electronics, Physical Chemistry, Organic Chemistry and Biochemistry, Materials Chemistry, Molecular Genetics, Molecular Medicine, Marine Research, Marine and Environmental Research, and Laser and Atomic Research and Development. The RBI scientists publish on average around 300 journal papers/year, in the internationally reviewed journals quoted in Current Contents (CC) secondary database. In parallel with fundamental research, applied research is performed in collaboration with other institutes and industrial units in various branches of production of goods. Co-operation occurs with a number of governmental institutions related to environmental protection, water management and protection, national defense, health care and food production. Institute includes the largest library for natural sciences in Croatia, a computing centre, animal facilities and a number of state-of-the-art experimental devices. Important aspect of dissemination of knowledge is participation in education at undergraduate and graduate level at the Universities of Zagreb, Osijek, Split and Rijeka, supervising B.Sc., M.Sc. and Ph.D. Thesis. International co-operation is essential aspect of knowledge dissemination, which enables up-to date research activities. One of the biggest RBI divisions, with about 100 employers, is the Center for Marine and Environmental Research (CMER).

Centre for Marine and Environmental Research, Rudjer Bošković Institute, Bijenička c. 54, P.O.Box 180, HR-10002 Zagreb, Croatia
Phone: **385-1-4680 215; Fax:**385-1-4680242,
Web: http://www.irb.hr

CMER was founded in 1969 and its research mission is focussed on physico-bio-geochemical processes in surface water and groundwater supplies. Since its foundation fundamental and applied research are going on in parallel, focused on marine, estuarine and freshwater systems. Multidisciplinary and interdisciplinary approaches are interconnected in research and education, especially in education of young scientists in Oceanology and in Environmental Protection and Nature Conservation. Long-term research programme of the Center, supported by the Ministry of Science and Technology of the Republic of Croatia, is focused on investigation of the biogeochemical cycles of inorganic and organic constituents and natural characteristics of the Adriatic Sea, estuarine and freshwater systems, evaluation of anthropogenic influence by modeling distribution and behavior of substances and their speciation in water and at natural phase boundaries, and assessment of the impact of inorganic and organic pollution on aquatic organisms and communities (ecological risk assessment) using methodology of exposure and effect biomarkers and monitoring health condition of the aquatic organisms. CMER has tradition and advanced role in developing electrochemical methods for research and pollution control in marine, estuarine and freshwater systems. CMER has well-equipped and specialized laboratory for radioactivity measurements in various types of environmental samples. CMER has specialized group for implementation of aqua-culture activities. Based on knowledge in information and communication technologies the professional team from CMER had the leading role in telematics application within the Danube Environmental Programme. Permanent staff at CMER consists of 56 Ph.D. researchers, 17 technicians and about 25 Ph.D. students and postdoctoral fellows. Laboratory and field research facilities exist in Zagreb and at the Adriatic coast, in Šibenik. Scientific staff of CMER has been continuously engaged in graduate and postgraduate courses at the University in different disciplines of natural science and technology. CMER views its future within the multilateral European and Mediterranean network of institutions promoting advanced environmental studies aimed to enhance the scientific knowledge needed to prevent and alleviate pollution caused by toxic chemicals and radionuclides.
CMER disposes with laboratory infrastructure and diverse instrumentation needed in environmental studies of inorganic and organic constituents and radionuclides:: a- and b-counter, g-spectrometer, counter for tritium, flame atomic absorption spectrometer, cold-vapor atomic absorption spectrometer, inductively coupled plasma with mass spectrometry, gas chromatographs equipped with various detectors such as mass spectrometric, electron capture ,thermoionic and flame ionization detectors, high performance liquid chromatographs equipped with spectrophotometric and spectrofluorimetric detectors, hyphenated system HPLC-tandem MS. A specialized equipment for equipment for digestion /extraction of inorganic (microwave-assisted digestion /extraction) and organic constituents (accelerated solvent extraction) is also available. In addition, more general equipment needed for sample storage, characterization and work-up is also available, including particle counter, total organic carbon (TOC) and POC analyzer, cooled Supraspeed centrifuge (up to 100 000xg), UV/VIS and fluorescence spectrophotometer, fixed wavelength microplate reader, deep-freezers at -80oC. We also have at our disposal a range of diverse electrochemical instruments for the analysis of metal and organic compounds, for studying physico-chemical processes at the phase boundary.

EU Project
Assessment of the selected POPs (PCBs, PCDDs/Fs, OPCs) in the atmosphere and water ecosystems from the waste materials generated by warfare in former Yugoslavia (EU-Project “Call for Balkan reintegration”; M. Picer)

International projects
Mitigation of environmental conseqences of the war in Croatia – risk assessment of hazardous chemical contamination (financial support from the Government of Norway; M. Ahel and G. Kniewald).
An integrated environmental monitoring system for Croatian freshwater, estuarine and coastal marine areas (financial support from the Research Council of Norway; K. Hylland, B. Raspor, S. Britvić, B. Hackenberger, G. Klobučar).
Ecosystem dynamics, marine chemistry, aquaculture and management in the Adriatic and north-Norwegian coastal zone (financial support from the Research Council of Norway; B. Ćosović and P. Wassmann).
Identification of bacterial structures important for bacterial adhesion using the electrochemical adhesion sensor (a bilateral Croatian-Swiss project between the Ruđer Bošković Institute and Swiss Federal Institute for Environmental Science and Technology funded by the Swiss programme SCOPES; V. Žutić and V. Svetličić).
Aromatic surfactants and emerging chemical contaminants – Analyses, occurrence and fate in the aquatic environment (ASECCO) (a bilateral Croatian-Swiss project between the Ruđer Bošković Institute and Swiss Federal Institute for Environmental Science and Technology funded by the Swiss programme SCOPES; W. Giger and M. Ahel).
Development of the new type of electrochemical sensor for research and control of reactive nanoparticles in aquatic environments (Project COCOP, bilateral Croatian-French cooperation between the Ruđer Bošković Institute and the Centre National de Recherche Scientifique,Paris, France; V. Žutić and V. Svetličić)

Development and innovative-technological projects
Conceptual model for biotechnological treatment of atrazine production waste water (D. Hršak)
Development of the new type of electrochemical sensor for monitoring of reactive microparticles (V. Žutić)
Integrated system for fairway surveillance on the Danube and Drava Rivers (I. Ružić)

Monitoring projects
- Radiological monitoring of nuclear power plant Krško (S. Lulić)
- Radiological water quality monitoring of the transboundary Danube river (S. Lulić)
- Information management for the transnational monitoring of the Danube river basin (I. Ružić)

On SARIB project four Laboratories from CMER are collaborating:
- Laboratory for biological effects of metals, head Dr. Biserka Raspor, raspor@rudjer.irb.hr
- Laboratory for aquaculture, head Dr. Emin Teskeredžić, etesker@rudjer.irb.hr
- Laboratory for organic biochemistry, head Dr. Marijan Ahel, ahel@rudjer.irb.hr
- Laboratory for molecular ecotoxicology, which includes the research goup of Dr. M. Picer (picer@rudjer.irb.hr) specialized in specific organic pollutants, head Dr. Smiljana Britvić, britvic@rudjer.irb.hr

The key staff for SARIB project from these Laboratories are:
- Dr. Biserka Raspor, Dr. Marijana Erk, M.Sc. Zrinka Dragun
- Dr. Emin Teskeredžić, Dr. Zlatica Teskeredžić
- Dr. Marijan Ahel, Dr. Senka Terzić, Iva Mikac, B.Sc.
- Dr. Smiljana Britvić, Dr. Tvrtko Smital, Dr. Mladen Picer, M.Sc. Tanja Kovač, Vedranka Hodak-Kobasić, B.Sc.

Role and Contribution of RBI to SARIB project
- Pore water and organic extracts from sediments and river water will be tested for AChE inhibition and MXR-inhibition.
- Health status of indigenous fish species from Sava River (determination of parasites, viruses, specific bacteria).
- Histological examination of fish tissue, blood analysis, biometry.
- Introduction of “passive sampling” devices for temporal accumulation of bioavailable fraction of metals (DGT) and classes of organic pollutants (SPMD), specifically PAH,s and PCB,s from water column.
- Analysis of extracts from “passive sampling” devices (DGT) on Cd,Cu,Zn,Pb,Hg,Ag,Ni,Mn,Fe.
- Analysis of extracts from “passive sampling” devices (SPMD) on PAH,s, PCB,s and specific organic pollutants which have (pre)mutagenic potential.
- Determination of biomarkers in fish liver tissue homogenates related to metal effect (metallothioneins), to PAH,s and PCB,s exposure (EROD activity) and effect (bioactivation potential; AMES test).
- Determination of metals (Cd,Cu,Zn,Pb,Hg,Ag,Ni,Mn,Fe) in supernatants in which metallothioneins are determined.
- Analysis on metal and PCB content in fish liver as target detoxification organ.
- Fish bile analysis on PAH content by fixed wavelength fluorescence, coupled with the advanced analytical tool for specific PAH compounds.

Experience of the group
Complementary expertise in chemical and biological knowledge exists within the research group from RBI involved in the design, proposal and realisation of SARIB project. Its competence is related to the aquatic environment and to:
- biogeochemistry of organic compounds with a special emphasis on persistent xenobiotic compounds. The two groups of target compounds, which have been intensively studied for more than 20 years, include organohalogen compounds and surfactants and their metabolites. More recently, the emphasis was put on emerging classes of aquatic contaminants and on the assessment of possible critical patterns leading to harmful effects in the environment. Furthermore, the recent focus is on polar compounds, such as aromatic surfactants and their metabolites and pharmaceutical compounds, which display an enhanced persistence and mobility in the aquatic environment. Analytical methods for the determination of natural and xenobiotic compounds in environmental samples have been based on highly specific analytical techniques such as high-resolution gas chromatography, high-performance liquid chromatography and mass spectrometry.
- research on metals and cellular biomarkers in the cytosol of target tissue from bivalve and fish species is primarily directed to indigenous organisms, in the areas of diverse anthropogenic activities. The biological effect measured as the level of inducible and metal-binding protein, metallothionein (MT), is related to the concentration of those metals which are know to induce MT under laboratory conditions. Beside that, additional abiotic and biotic parameters are taken into account in order to get better insight into the role of baseline MT level in indigenous indicator aquatic organisms.
- multixenobiotic resistence mechanism as a biomarker of environmental quality, focused on the identification of detoxification and/or defense mechanisms that are present in taxonomicaly various classes of marine and freshwater organisms. Present activities in environmental toxicology include: investigations of metabolic pathways of mutagenic and/or carcinogenic substances in water; determination of the mixed function monooxygenases (MFO) activity in fish; analysis of the estrogen activity of chemicals and/or environmental samples; investigation of the presence and function of multixenobiotic resistance (MXR) mechanism in aquatic organisms; development of in vitro and in vivo bioassays for the determination of MXR inhibitors in environmental samples. Included are enzymatic analysis, Ames genotoxicity test, cell and yeast culture techniques, immunochemical determination of protein titer, as well as measurements of transmembrane protein transport activity in mussels and fish.
- protection of biocenotic balance in aquaculture, determining the health status of fish, implementation and development of aquaculture technologies..
The research team from CMER/RBI disposes with the necessary chemical and biochemical instruments and equipment for the performance of the proposed research activities within SARIB project.

Dr. Biserka Raspor, leader of the WP3, senior scientist, employed at RBI since 1968. Head of the Laboratory for biological effects of metals. Supervisor of postograduate and dosctoral students. In 1968 graduate in chemistry, faculty of Chemical Engineering. M.Sc. degree in physical chemistry in 1970 and Ph.D. degree in chemistry at the Faculty of Natural Sciences, University of Zagreb. Published 40 papers quoted by Current Content (CC) secondary base, 3 chapter in international book editions on metals and their speciation in aquatic systems, additional research papers in the Proceedings of international conferences, professional papers. Co-operation with School of Chemistry, Bristol, GB, Research Center in Juelich, Germany, with University in Bremen, Germany. Leading the projects with NIST, UNEP. Field of expertise is chemical speciation of Cd, Pb, Cu, Zn in marine and freshwater systems; physico-chemical processes at the mercury/eletrolyte interface; chemical reactivity of metallothioneins (MT); methods of MT isolation, determination; metal analysis in biological and biochemical samples.

Dr. Marijana Erk, research associate, employed at RBI since 1991. Graduate at Faculty for Education in Osijek in 1991, M.Sc. degree in Oceanology in 1995, Ph.D. in chemistry in 2000 both at Faculty of Natural Sciences, University of Zagreb. Published 10 CC quoted research papers. Field of expertise is related to the investigation of metal binding properties by MT, improvement and development of methods for MT determination. Leader of the research project for young scientists supported by Ministry of Science Republic Croatia. Participant of international Becpelag project and the National project “Adriatic”. Post-doctoral fellow at NIVA, Oslo, Norway.

M.Sc. Zrinka Dragun, assistant, employed at RBI since December 2002 as Ph.D. Student. Graduated in 1996 on Faculty of Farmacy and Biochemistry. University of Zagreb. M.Sc. degree from toxicology in 2001 at Faculty of Natural Sciences, University of Zagreb. Published 1 research paper quoted by CC. Expertise will be directed to determination of metals in biological and biochemical samples related to MT level.

Dr. Marijan Ahel, senior scientist, employed at RBI since 1975. Presently, head of the Laboratory for biogeochemistry of organic compounds, CMER/RBI. Graduate in Biotechnology 1975, M. Sc. in Marine Sciences 1983 and Ph.D. in Chemistry from the Rudjer Bošković Institute in Zagreb in 1987. Published over 50 papers in journals cited by SCI. Leader of several national and international projects. Lecturer of Analytical chemistry and Analytical chemistry of organic pollutants at the University of Zagreb. His main interest is in the development of novel analytical methods for the determination of natural and xenobiotic compounds in environmental samples using highly specific analytical techniques such as high-resolution gas chromatography, high-performance liquid chromatography and mass spectrometry. Regarding xenobiotics, the emphasis is on emerging classes of aquatic contaminats and on the assessment of possible critical patterns leading to harmful effects in the environment. Recent focus is on polar compounds, such as aromatic surfactants and their metabolites and pharmaceutical compounds, which display an enhanced persistence and mobility in the aquatic environment.

Dr. Senka Terzić, research associate, employed at RBI since 1988. Graduate in Biotechnology in 1988, Ms. Sci. in Marine Sciences in 1990 and Ph.D. in Chemistry from the Faculty of Natural Sciences of the University of Zagreb in 1996. She was involved in several international projects dealing with biogeochemistry of organic compounds in the aquatic environment. Her main interest is in the analysis and biogeochemical behaviour of selected classes of xenobiotic compounds, such as surfactants and their metabolites, as well as in studying phenomena associated with potentially harmful changes of carbon cycling in eutrophic marine environments.

Mrs. Iva Mikac, Ph.D. student, employed at RBI since January 2003. Graduate in chemistry at the Faculty of Sciences of the University of Zagreb in 2002. She was involved in the monitoring program of the Adriatic Sea using HPLC-based methodology. Her research focus will be on environmental behaviour and fate of polycyclic aromatic hydrocarbons.

Dr. Smiljana BRITVIĆ, higher research associate at the CMER/RBI, Zagreb, Croatia. Head of the LME. Ph.D. in Biology-Oceannology in 1980, Faculty of Science, University of Zagreb, Croatia. Lecturer of Ecotoxicological effects of contaminants at the University of Zagreb. She was engaged in many applied projects of the CMER aimed at the assessment of risk from Nuclear Power plant, quality of drinking waters, quality of Sava River waters, quality of Rijeka Bay waters. Leader the project of FAO UNEP: “The environmental implications of the multixenobiotic resistance mechanism expressed in marine invertebrates”, and the Open Society Institute – Croatia Project: “Bakar Bay: Monitoring of Recovery Using Biomarkers”, and as a co – worker in about 10 international Projects (US EPA, Germany – Croatia bilateral, UNEP MAP etc.). Author and co-author of more than 40 SCI papers.

Dr. Tvrtko SMITAL, research associate at the CMER/RBI. Main investigator and project leader in Laboratory for Molecular Ecotoxicology. Ph.D. in Biology-Ecotoxicology in 1999, Faculty of Science, University of Zagreb, Croatia. Lecturer of Ecotoxicological effects of contaminants at the University of Zagreb and Ecotoxicology at the University of Osijek. Apart from other techniques present in LME his experience especially includes investigation of the presence and function of multixenobiotic resistance (MXR) mechanism in aquatic organisms. Author and co-author in 11 SCI papers and 1 book chapter.

Dr. sci. Mladen Picer, senior scientist at the CMER/RBI. Main research interest and expertise has in investigation instrumental methods for POPs determination in environmental samples and their biogeochemical fate in the environment. Since 2002 is scientific coordinator of APOPSBAL ICA-2 CT 2002- 10007 Project (Call Identifier INCO-COPERNICUS -ICFP501A2PR02).

M. sci. Tanja Kovač, is an environmental biotechnologist and specialist in the field of investigation instrumental methods for chlorinated hydrocarbons determination (especially PCBs by GC) in environmental samples and their interaction within aquatic organisms.

Vedranka Hodak - Kobasić obtained her B. Sc. Degree 2001, from Food Technology and Biotechnology Faculty , University of Zagreb.

Dr. Emin Teskeredžić, senior scientist and the head of the Laboratory for aquaculture at CMER/RBI. Coordinator of several national and international projects related to fish health and aqua-culture. In 1981 stayed for several months at Hokkaido Fish Hatchery, Sapporo, Japan. In the period from 1989-1990 visiting scientist at Department of Fisheries and Oceans, West Van Lab, Vancouver, Canada. Published about 30 peer-reviewed papers and attended over 40 conferences. Supervisor of several B.Sc., M.Sc. and Ph.D. Thesis.

Dr. Zlatica Teskeredžić, senior scientist involved in fish nutrition research. She has been a visiting scientist at Department of Fisheries and Oceans, West Van Lab, Vancouver Canada in 1989/1990 and 1990/1991 working on a Project to replace a fishmeal with a rapeseed protein concentrate in feed for rainbow trout. Published about 40 peer-reviewed papers and attended over 35 conferences. Supervised a number of M.Sc. and Ph.D. Thesis.

Relevant Research papers by RBI group published in the period 1998-2003 and quoted by CC

Ahel, Marijan ; Mikac, Nevenka ; Ćosović, Božena ; Prohić, Esad ; Soukup, Vesna. The impact of contamination from a municipal solid waste landfill (Zagreb, Croatia) on underlying soil. // Water science and technology. 37 (1998), 8; 203-210.
Ahel, Marijan ; Terzić, Senka Pigment signatures of Phytoplankton Dynamics in the Northern Adriatic. // Croatica chemica acta. 71 (1998), 2; 199-215.
Ahel, Marijan ; Tepić, Nataša. Distribution of polycyclic aromatic hydrocarbons in a municipal solid waste landfill and underlying soil. Bulletin of Environmental Contamination & Toxicology. 65 (2000.), 2; 236-243.
Ahel, Ivan ; Tepić, Nataša ; Britvić, Smiljana ; Ahel, Marijan. Assessment of organic pollution in the Jakuševec landfill (Croatia) by biomarker and chemical methods. Fresenius Environmental Bulletin. 9 (2000); 726-733.
Ahel, Marijan ; Giger, Walter ; Molnar, Eva ; Ibrić, Slavica. Determination of nonylphenol polyethoxylates and their lipophilic metabolites in sewage effluents by normal-phase high-performance liquid chromatography and fluorescence detection. Croatica chemica acta. 73 (2000), 1; 209-227.
Ahel, Marijan ; Molnar, Eva ; Ibrić, Slavica ; Giger, Walter. Estrogenic metabolites of alkylphenol polyethoxylates in secondary sewage effluents and rivers. Water Science & Technology. 42 (2000), 7-8; 15-22.
Britvić, Smiljana ; Kurelec, Branko. The effect of inhibitors of multixenobiotic resistance mechanism on the production of mutagens by Dreissena polymorpha in waters spiked with premutagens. // Aquatic toxicology. 47 (1999), 2; 107-116.
Čož-Rakovac, Rozelindra ; Teskeredžić, Emin ; Hacmanjek, Mato ; Tomec, Marija ; Topić Popović, Natalija ; Strunjak-Perović, Ivančica. Seawater challenge test and body composition of Coho salmon (Onchorynchus kisutch). // Veterinary Medicine - Czech. 44 (1999), 12; 365-371.
Ćosović, Božena ; Ciglenečki, Irena ; Viličić, Damir ; Ahel, Marijan. Distribution and seasonal variability of organic matter in a small eutrophicated salt lake.Estuarine, Coastal and Shelf Science. 51 (2000); 705-715.
Erk, Marijana; Ivanković, Dušica; Raspor, Biserka; Pavičić, Jasenka. Evaluation of different purification procedures for the electrochemical quantification of mussel metallothioneins. // Talanta. 57 (2002); 1211-1218.
Erk, Marijana ; Raspor, Biserka. Anodic stripping voltammetry in the complexation study of the peptide Lys-Cys-Thr-Cys-Cys-Ala (56-61)MT I and cadmium: application in determination of complexing capacity and stability constant . // Journal of Electroanalytical Chemistry. 502 (2001), 1-2; 174-179.
Erk, Marijana; Raspor, Biserka. Interference of Pb leaching from the pH electrode on Cd-metallothionein complex. // Analytica Chimica Acta. 442 (2001); 165-170.
Erk, Marijana ; Raspor, Biserka. Advantages and disadvantages of voltammetric method in studying cadmium-metallothionein interactions. // Cellular and Molecular Biology. 46 (2000), 2; 269-281.
Erk, Marijana ; Raspor, Biserka. Electrochemical study on Cd binding to metallothioneins isolated from the mussel, Mytilus galloprovincialis. // Journal of electroanalytical chemistry . 466 (1999), 1; 75-81.
Erk, Marijana ; Musani, Ljerka ; Raspor, Biserka. The interactions of 54Mn with humic acids in freshwater systems. // Water research. 32 (1998), 6; 1753-1758.
Erk, Marijana ; Raspor, Biserka. Evaluation of cadmium-metallothionein stability constants based on voltammetric measurements. // Analytica chimica acta. 360 (1998); 189-194.
Fajon, Céline ; Cauwet, Gustave ; Lebaron, Philippe ; Terzić, Senka ; Ahel, Marijan ; Malej, Alenka ; Mozetič, Patricija ; Turk, Valentina. The accumulation and release of polysaccharides by planktonic cells and the subsequent bacterial response during a controlled experiment. // FEMS microbiology ecology. 29 (1999), 4; 351-363.
Filipović, Vlatka; Raspor, Biserka. Metallothionein and metal levels in cytosol of liver, kidney and brain in relation to growth parameters of Mullus surmuletus and Liza aurata from the Eastern Adriatic Sea. Water Research. (2003) in press.
Ivanković, Dušica; Pavičić, Jasenka; Kozar, Sonja; Raspor, Biserka. Multiple forms of metallothionein from the digestive gland of naturally occurring and cadmium exposed mussels, Mytilus galloprovincialis. // Helgoland Marine Research. 56 (2002); 95-101.
Ivanković, Dušica ; Pavičić, Jasenka ; Raspor, Biserka ; Falnoga, Ingrid ; Tušek-Žnidarič, Magda. Comparison of two SH-based methods for estimation of metallothionein level in the digestive gland of naturally occurring mussels, Mytilus galloprovincialis. // International Journal of Environmental Analytical Chemistry 83 (2002); 219-231.
Jeličić, Ivana; Ahel, Marijan. Occurance of phenazone analgesics and caffeine in Croatian municipal wastewaters. // Fresenius Environmental Bulletin. 12 (2003), 1; 46-50.
Jukić, Silvana ; Miletić, Ivana ; Anić, Ivica ; Britvić, Smiljana ; Osmak, Maja ; Sistig, Suzana. The mutagenic potential of AH+ and AH26 by salmonella/microsome assay. // Journal of endodontics. 26 (2000), 6; 321-324.
Kurelec, Branko ; Smital, Tvrtko ; Pivčević, Branka ; Eufemia, Nancy ; Epel, David. Multixenobiotic resistance, P-glycoprotein, and chemosensitizers. // Ecotoxicology. 9 (2000); 307-327.
Kurelec, Branko ; Britvić, Smiljana ; Pivčević, Branka ; Smital, Tvrtko. Fragility of multixenobiotic resistance in aquatic organisms enhances the complexity of risk assessment. // Marine environmental research. 46 (1998), 1-5; 415-419.
Mikac, Nevenka ; Ahel, Marijan ; Kozar, Sonja ; Prohić, Esad. Distribution of contaminantsin soil and groundwater below and downstream of the Jakuševec landfill (Croatia) . // Fresenius environmental bulletin. 8 (1999), 11-12; 645-653.
Mikac, Nevenka ; Ćosović, Božena ; Ahel, Marijan ; Andreis, Svjetlana ; Tončić, Zdenka. Assessment of groundwater contamination in the vicinity of a municipal solid waste landfill (Zagreb, Croatia). // Water science and technology. 37 (1998), 8; 37-44.
Naudin, Jean-Jacques ; Cauwet, Gustave ; Fajon, Celine ; Oriol, Louise ; Terzić, Senka ; Devenon, Jean-Luc ; Broche, Pierre. Effect of mixing on microbial communities in the Rhone River plume. Journal of Marine Systems. 28 (2001); 203-227.
Osmak, Maja ; Brozović, Anamaria ; Ambriović-Ristov, Andreja ; Hadžija, Mirko ; Pivčević, Branka ; Smital, Tvrtko. Inhibition of apoptosis is the cause of resistance to doxorubicin in human breast adenocarcinoma cells. // Neoplasma. 45 (1998), 4; 223-230.
Picer, Mladen ; Kovač, Tatjana ; Britvić, Smiljana ; Picer, Nena. The chemical and biogenotoxic characterization of organic xenobiotics in aquatic sediment materials 1. The application and comparison of chemically nonspecific and biogenotoxic mathods. // Chemosphere. 44 (2001), 8; 1673-1683.
Picer, Mladen ; Kovač, Tatjana ; Britvić, Smiljana ; Picer, Nena. The chemical and biogenotoxic characterization of organic xenobiotics in aquatic sediment materials 1. The application and comparison of chemically nonspecific and biogenotoxic mathods. // Chemosphere. 44 (2001), 8; 1673-1683.
Picer, Mladen. DDTs and PCBs in the Adriatic Sea. // Croatica chemica acta. 73 (2000), 1; 123-186.
Plavšić, Marta; Terzić ,Senka; Ahel, Marijan ;van den Berg, C.M.G. Folic acid in coastal waters of the Adriatic sea. // Marine and Freshwater Research. 53 (2002), 8; 1245-1252.
Ramljak, Sanja ; Hackenberger, Branimir ; Smital, Tvrtko ; Britvić, Smiljana. EVALUATION OF THE GENOTOXIC AND CYTOCHROME P450 MONOOXYGENASE-INHIBITORY POTENTIAL OF DICURAN ON PROCARYOTIC AND EUCARYOTIC TEST SYSTEMS. // Journal of Environmental Science and Health. Part B - Pesticides, Food Contaminants, and Agricultural Wastes. B35 (2000), 6; 751-770.
Raspor, Biserka. Elucidation of the mechanism of the Brdička reaction. // Journal of electroanalytical chemistry. 503 (2001), 1-2; 159-162.
Raspor, Biserka ; Paić, Marina ; Erk, Marijana. Analysis of metallothioneins by the modified Brdička procedure. // Talanta. 55 (2001); 109-115.
Raspor, Biserka ; Kozar, Sonja ; Pavičić, Jasenka ; Jurič, Dušica. Determination of the cadmium and copper content inherent to metallothionein. // Fresenius journal of analytical chemistry. 361 (1998); 197-200.
Smital, Tvrtko; Sauerborn, Roberta. MEASUREMENT OF THE ACTIVITY OF MULTIXENOBIOTIC RESISTANCE MECHANISM IN THE COMMON CARP Cyprinus carpio. // Marine Environmental Research. 54 (2002); 449-453.
Smital, Tvrtko ; Sauerborn, Roberta ; Pivčević, Branka ; Krča, Sanja ; Kurelec, Branko. Interspecies differences in P-glycoprotein mediated activity of multixenobiotic resistance mechanism in several marine and freshwater invertebrates. // Comparative Biochemistry and Physiology C. 126 (2000); 175-186.
Smital, Tvrtko ; Kurelec, Branko. The activity of multixenobiotic resistance mechanism determined by rhodamine B-efflux method as a biomarker of exposure . // Marine environmental research. 46 (1998), 1-5; 443-447.
Sondergaard, Morten ; Williams, Peter J. le B., Cauwet, Gustave ; Riemann, Bo ; Robinson, Carol ; Terzić, Senka ; Woodward, E. Malcolm S. ; Worm, Jakob. Net accumulation and flux of dissolved organic carbon and dissolved organic nitrogen in marine plankton communities. // Limnology & Oceanography. 45 (2000), 5; 1097-111.
Terzić, Senka ; Ahel, Marijan. Distribution of carbohydrates during a diatom bloom in the northern Adriatic. // Croatica chemica acta. 71 (1998), 2; 245-262.
Terzić, Senka ; Ahel, Marijan ; Cauwet, Gustave ; Malej, Alenka.Group-specific phytoplankton biomass/dissolved carbohydrate relationships in the Gulf of Trieste (Northern Adriatic). // Hydrobiologia. 363 (1998); 191-205.
Topić Popović, Natalija ; Hacmanjek, Mato ; Teskeredžić, Emin. Health status of rudd (Scardinius erythrophtalmus hesperidicus H.) living in the lake Vrana on the island of Cres.. // Journal of Applied Ichthyology. 17 (2001); 43-45.